Bacterial leaf steak (BLS) brought on by Xanthomonas oryzae pv. oryzicola (Xoc) is a devastating illness in rice production. The resistance to BLS in rice is a quantitatively inherited trait, of which the molecular mechanism is nevertheless unclear. It has been proved that xa5, a recessive bacterial blast resistance gene, is the most achievable candidate gene of the QTL qBlsr5a for BLS resistance. To study the molecular mechanism of xa5 function in BLS resistance, we made transgenic lines with RNAi of Xa5 (LOC_Os05g01710) and applied RNA-seq to analyze the transcriptomes of a Xa5-RNAi line and the wild-variety line at 9 h following inoculation with Xoc, with the mock inoculation with water as manage. The final results showed that Xa5-RNAi could (1) enhance the resistance to BLS as anticipated from xa5 (two) alter (primarily up-regulate) the expression of hundreds of genes, most of which had been associated to illness resistance and (three) significantly boost the response of thousands of genes to Xoc infection, particularly of the genes involved in cell death pathways, suggesting that xa5 displays BLS resistance impact almost certainly primarily simply because of the enhanced response of the cell death-associated genes to Xoc infection.